true RIKEN BRC <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/feb_2012_mm" target="_blank">Mouse of the Month Feb 2012</A><br><a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/01626_GB.pdf">Genetic Background</a> RIKEN BRC D (more than 6 months) RBRC01626 D（6か月以上） The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the Department of Experimental Genome Research. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. This strain was generated in C57BL/6 mice by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University (RBRC00267 C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb). The mice were then backcrossed to BALB/cANMs (RBRC01624 C.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc), C3H/HeNCrlCrlj (RBRC01624 C3.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc), MSM/MsRbrc (RBRC01626 MSM;B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc) and DBA/2CrSlc (RBRC02889 D2.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Ny), respectively. Congenic strains of BALB/c, C3H and MSM background were developed by RIKEN BioResource Center, and DBA/2 congenic strain was developed by Naoki Yamamoto, Fijita Health University. オワンクラゲの蛍光蛋白を全身の細胞で発現する遺伝子導入マウス。480 nmの励起光のもとで全身が緑色の蛍光を発するので別名「グリーンマウス」とも呼ばれる。受精卵から細胞分裂により個体が形成される過程で細胞がどのように分化して運命が決定されていくのか、その細胞系譜を生体の中で追跡するのに有用な系統。発生研究、がん研究、再生医学研究の分野で利用される。トランスジーンの挿入位置は14D1。 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol> This transgenic mice carry an EGFP (enhanced green fluorescence protein) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. Both homozygous and hemizygous transgenic mice express GFP in all over the body. This strain is useful for detection of cell fate in transplantation and developmental studies. MSM.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc MSM.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc 条件を付加する。<br>利用者は事前に大阪大学微生物病研究所遺伝子機能解析分野の提供承諾書を得る。非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。研究成果の公表にあたって遺伝子機能解析分野の指定する文献を引用する。5年経過後も使用を希望する時は改めて寄託者から承諾を得るものとする。